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The research group aims to detect the molecular and cellular processes that underlie so-called immuno-vascular interactions under inflammatory conditions. In order to understand, for example, an infection and its corresponding immune response, the scientists look at the process from different angles.

One of the imaging techniques that the researchers use is fluorescence microscopy. This form of light microscopy includes, among others, light sheet fluorescence microscopy (LSFM). LSFM enables the scientists to determine information on different classes of molecules and their spatial distribution patterns at the same time. In addition to imaging techniques like LSFM, the researchers also work with confocal laser scanning microscopy (CLSM) and two-photon laser scanning microscopy (TPLSM). They enable a three-dimensional analysis of biological samples from a cellular to sub-cellular level.

The researchers conduct studies on animal and human samples, take measurements of intact organs and integrate artificial intelligence into their image analyses.

Um die Knochenhomöostase zu gewährleisten, werden die Knochenoberfläche – das Periost - sowie der kortikale Knochen mithilfe verschiedener Blutgefäßtypen wie feinen Kapillaren (rot) und großen Arterien (grün und rot) versorgt.

To ensure bone homeostasis, the bone surface – the periosteum – and the cortical bone are supplied by different types of blood vessels, such as fine capillaries (red) and large arteries (green and red).

© ISAS / Anika Grüneboom

Verteilung von Lysosomen (rot) in humanen mesenchymalen Stammzellen. Das Zytoskellet ist grün und die Zellkerne sind blau dargestellt.

Distribution of lysosomes (red) in human mesenchymal stem cells. The cytoskeleton is shown in green and the nuclei are blue.

© ISAS / Anika Grüneboom

Farbkodierung der räumlichen Orientierung von Aktinfasern in humanen Fibroblasten.

Colour coding of the spatial orientation of actin fibers in human fibroblasts.

© ISAS / Anika Grüneboom

Identifizierung von resorptiv aktiven (rot, grün, weiß) und inaktiven Osteoklasten (grün, weiß) an der Wachstumsfuge und Trabekeln.

Identification of resorptively active (red, green, white) and inactive osteoclasts (green, white) at the growth plate and trabeculae.

© ISAS / Anika Grüneboom

Hepatische Makrophagen (rot) interagieren primär mit sinusoidalen Endothelzellen (grün) während an der Zentralvene (weiß) kaum Immunzell-Interaktionen zu beobachten sind.

Hepatic macrophages (red) primarily interact with sinusoidal endothelial cells (green), while hardly any immune cell interactions are observed at the central vein (white).

© ISAS / Anika Grüneboom

Die Färbung der neutralen Lipide (grün) und der Basalmembran (rot) ermöglicht den Nachweis von Lipidtropfen, die entlang des Endomysiums eines Patienten mit kongenitalem myasthenischem Syndrom aufgereiht sind. Die Mitochondrien (weiß) weisen geringere Mengen an neutralen Lipiden auf als das Endomysium.

The staining of neutral lipids (green) and basement membrane (red) allows the detection of lipid droplets lined up along the endomysium of a patient with congenital myasthenic syndrome. The mitochondria (white) have lower amounts of neutral lipids than the endomysium.

© ISAS / Anika Grüneboom

Lokalisation von Osteoklasten (grün) entlang der ossären Blutgefäße (rot) im murinen Mandibel.

Localisation of osteoclasts (green) along osseous blood vessels (red) in the murine mandible.

© ISAS / Anika Grüneboom

Lichtblatt-Fluoreszenzmikroskopische Aufnahme der Immunzell-Infiltration (rot-orange) in einem murinen Herzen (blau) nach Herzinfarkt.

Light sheet fluorescence microscopy of immune cell infiltration (red-orange) in a murine heart (blue) after myocardial infarction.

© ISAS / Anika Grüneboom

Lichtblatt-Fluoreszenzmikroskopische Aufnahme der räumlichen Verteilung verschiedener Makrophagen-Populationen (blau und grün) entlang der Blutgefäße und Glomeruli (rot) in einer murinen Niere.

Light sheet fluorescence microscopy of the spatial distribution of different macrophage populations (blue and green) along blood vessels and glomeruli (red) in a murine kidney.

© ISAS / Anika Grüneboom

Infiltration von Immunzellen (rot) und entzündungs-getriebene Angiogenese von Blutgefäßen (grün und weiß) in einem murinen Kniegelenk während rheumatoider Arthritis.

Infiltration of immune cells (red) and inflammation-driven angiogenesis of blood vessels (green and white) in a murine knee joint during rheumatoid arthritis.

© ISAS / Anika Grüneboom

Infiltration von Immunzellen (rot) und entzündungs-getriebene Angiogenese von Blutgefäßen (grün und weiß) in einem murinen Kniegelenk während rheumatoider Arthritis.

Infiltration of immune cells (red) and inflammation-driven angiogenesis of blood vessels (green and white) in a murine knee joint during rheumatoid arthritis.

© ISAS / Anika Grüneboom

Die Blutgefäßarchitektur der murinen Tibia umfasst sowohl venöse Sinusoide (rot) als auch Arterien (gelb) im Knochenmark, sowie arterielle (gelb) und venöse (rot) Transkortikalgefäße, die das Knochenmark durch den kortikalen Knochen hindurch mit dem peripheren Gefäßsystem verbinden.

The blood vessel architecture of the murine tibia includes both venous sinusoids (red) and arteries (yellow) in the bone marrow, as well as arterial (yellow) and venous (red) transcortical vessels connecting the bone marrow through the cortical bone to the peripheral vasculature.

© ISAS / Anika Grüneboom

Highlights

Scientist / Postdoc (m/f/d): Bioimaging

12th January 2024

“My research is literally hard work"

Darleen Hüser is looking for the immuno-cellular fingerprint of rheumatoid arthritis. In this interview, the doctoral student reveals her razor-sharp research and why she needs different microscopes.

Das Porträt zeigt ISAS-Doktorandin Darleen Hüser aus der Arbeitsgruppe Bioimaging.
21st December 2023

Science Slam: Humorous Science Communication Is Fun for Everyone

Talking lab equipment, artificial intelligence and expertise from the North Pole - this colourful mix of topics characterised the latest Science Slam at the institute. Four ISAS employees demonstrated with their specialist knowledge and plenty of humour how science communication can be fun for everyone involved.

Luisa Becher fotografiert die vier Teilnehmenden des ISAS Science Slam.
1st December 2023

Bone research: ISAS participates in the new "DIONE" Collaborative Research Centre

The nationwide project recently funded by the German Research Foundation is focussing on inflammation-induced bone loss. Researchers from Dortmund, Dresden, Erlangen/Nuremberg and Ulm want to find out exactly how inflammatory diseases - such as rheumatoid arthritis or intestinal diseases - damage bones. Among other things, their research aims to help identify new therapies for skeletal-associated diseases.

Bone structur with osteoporosis.
23rd September 2022

Hand in Hand for Successful Publications

Dr Rita Strack revealed the secret of successful publications during her talk at ISAS. Even for established scientists, much of what the Nature Methods senior editor revealed in Dortmund was new.

Group photo visit of Dr Strack.
16th September 2022

Differences are Critical

What do sex-specific differences mean for health research? What role do they play in applied basic research? Five ISAS scientists with different research focuses have an answer to these questions.

Female symbol, unequal, male symbol.
15th July 2022

More Than Jewellery: Silver as Protection against Implant Infections

Supporting or replacing bodily functions is possible thanks to implants - but only if they do not lead to bacterial infections. To prevent the latter, Kaja Reiffert is investigating the antimicrobial effect of silver on the surface of implants.

Portrait Kaja Reiffert.
14th June 2022

Why are there several vaccines against COVID-19, but yet none against AIDS?

Immunologist Prof Dr Matthias Gunzer explains, which characteristics of the HI virus are responsible for the fact that vaccine development takes longer than with Covid-19.

Portrait von Prof. Dr. Matthias Gunzer.

Projects

3D Molecular Pathology

The aim of the work carried out by researchers in the »3D molecular pathology« project is to gain a better understanding of the influence of inflammatory cells on the course of diseases that trigger massive immune reactions.

Imaging of Large Tissues

Researchers on the »Imaging of Large Tissues« project are developing a workflow to combine the various microscopy imaging methods and analytical, mass spectroscopy methods.

Synthesis, Structure & Biological Effects of Ultrasmall (1–2 nm) Bimetallic Silver-Platinum Nanoparticles

In the »Synthesis, Structure & Biological Effects of Ultrasmall (1–2 nm) Bimetallic Silver-Platinum Nanoparticles« project, ISAS researchers are examining the antimicrobial activities of nanoparticles.

TRR 332 – Neutrophil Granulocytes: Development, Behaviour & Function

At ISAS, scientists in the subproject »Phagocytic crosstalk between neutrophils and macrophages« investigate how immune cells of the type of phagocytes – in specific neutrophil granulocytes and macrophages – communicate with one another.

Smart Human-in-the-loop Segmentation

Scientist working on the project »Smart Human-in-the-loop Segmentation« aim to develop a powerful deep learning model that is trained with a minimum amount of human effort.

Team

Prof. Dr. Anika Grüneboom

Research Group Leader

Department: Biospectroscopy

Research group: Bioimaging

Portrait von Prof. Dr.  Anika Grüneboom.

Darleen Hüser

Research Associate

Department: Biospectroscopy

Research group: Bioimaging

Portrait von  Darleen Hüser.

Lara Melina Janz

Intern

Department: Biospectroscopy

Research group: Bioimaging

Luisa Röbisch

Technical Assistant

Department: Biospectroscopy

Research group: Bioimaging

Portrait von  Luisa Röbisch.

Dr. Martin Stenzel

Research Associate

Department: Biospectroscopy

Research group: Bioimaging

Portrait von Dr. Martin Stenzel.

Flora Weber

Research Associate

Department: Biospectroscopy

Research group: Bioimaging

Portrait von  Flora Weber.