Skyline Course 2023 – Register Now

Dortmund, 2nd December 2022

A training course on the open source software Skyline is taking place at ISAS in Dortmund from 20 to 23 March 2023. The course on using the software for (un)targeted proteomics and metabolomics, lipidomics and glycomics is aimed at beginners and advanced users.

Topics

Day 1 – Introduction to Targeted Quantitative Proteomics & Skyline, SRM Assay Development

Day 2 – Parallel Reaction Monitoring (PRM), Indexed Retention Time & Absolute Quantification

Day 3 – MS1 Filtering, DIA Data Analysis & Further Advanced Topics

Day 4 – Small Molecules Data Analysis

The registration fee – 540 euros (academic) and 810 euros (industry) – includes course materials, refreshments, lunch and one dinner. Participants need to bring their own notebooks to the course (OS Windows or a virtual Windows machine, at least 8 GB RAM, admin rights).

Registration until January 20

Registration is possible via this form, either by e-mail to skyline2023@isas.de or fax to +49 (0) 2 31.13 92-200, until Friday, January 20, 2023. ISAS will consider registrations following the order in which they were received. The course will take place with a minimum registration of 20 participants.

Further articles

3 Questions for Neele Rottmann

In the lab, Neele Rottmann prepares human and animal samples for mass spectrometry analysis. As part of the HI-FIVE project, she is helping to identify changes at the protein level in heart failure. Why does she spend so much time in the lab working on separations? The technical assistant shares the answer in this interview.

Neele Rottmann is wearing a white lab coat and purple gloves and is operating a mass spectrometry analyser in a laboratory. Next to her is a liquid chromatography system connected to a mass spectrometer via several tubes. There are numerous sample vials on the instrument. As she places a sample into the system, she looks into the camera.

3 Questions for Lukas Fu

How can AI support the analysis of biomedical images? This was the question Lukas Fu explored during his school internship at ISAS. The 15-year-old also successfully completed his first programming project.

Lukas Fu, a 15-year-old with short black hair. He is wearing glasses and is sitting on a low wall.

Separating Fluorescence Signals Faster and More Precisely with AI

When different fluorescence signals overlap during microscopy, their clear assignment to specific biological structures becomes difficult. To address this common problem, Dr Davide Panzeri is developing AI-based signal separation methods. The biophysicist has been awarded a Marie Skłodowska-Curie Fellowship by the European Union for his promising research project.

Passionate Research, Shaping the Future

How do GRK5 inhibitors work at the cellular level and within the living organism? Biologist Dr Susanne Grund is working with her colleagues at HI-FIVE on a new therapeutic approach for heart failure. In this portrait, she describes what her day-to-day work looks like, balancing research on human stem cells with studies on mice.

ISAS BFF Uploader

What are you doing at ISAS, Theresa?

Why do mice undergo ultrasound examinations of their hearts? What does a typical working day in the lab involve for a technical assistant in the HI-FIVE research project ? And what challenges does the job present? Theresa Pietz provides fascinating insights into her duties, motivations and experiences working with animals.

Study with Heart: A Doctor Bridging Clinic & Laboratory

What is actually happening behind the scenes of a patient trial? Anna Ruzhyna is a clinical research associate who looks after participants with heart failure as part of the HI-FIVE project. She explains how her work helps to develop new therapeutic approaches for the treatment of heart failure.

Laboratory Meets Clinic: Understanding Heart Function with Stem Cells

How can new therapeutic approaches for heart failure be developed more effectively? Stem cell research plays a central role in the HI-FIVE project. In this interview, PD Dr Anna Klinke (Herz- und Diabeteszentrum NRW) talks about her work between the lab and the clinic. She explains how the versatility of stem cells enables heart and vascular cells to be obtained from individual patients' blood and why this is an important step in investigating the specific effects of new active substances.

What are you doing at ISAS, Kevin?

What changes take place in heart muscle cells when a left ventricular assist device is used? ISAS doctoral student Kevin Hau (28) is tackling this question by researching the consequences of heart attacks using various omics analyses.