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Dortmund, 4th May 2023

Damit die Proteine im Massenspektrometer analysiert werden können, trennt Joy Amrei Brummel sie an der Zentrifuge aus den Proben heraus.
© ISAS - Joy Amrei Brummel purifies the thrombocytes at the centrifuge and isolates the cells for analysis in the mass spectrometer.

Joy Amrei Brummel (24) is studying Chemical Biology at TU Dortmund. Since her initiative application at ISAS in May 2022, she has been working as a student assistant in the Proteomics research group alongside her university studies. She is also writing her master’s thesis at ISAS. To learn more about her work in the laboratory, the editorial team asked her to complete the following sentences.

  • As a student assistant in the Proteomics research group…

    I help prepare samples for mass spectrometric analysis. I also assist my colleagues in the lab. For example, I take inventory of the samples in storage or prepare buffers, chemical solutions that maintain the stable pH value in a sample. It’s great to apply the methods you learned about in theory during your studies in an intensive and practical manner here.

  • My bachelor thesis deals with…

    the topic of thrombosis. More precisely, it’s about the mass spectrometric analysis of proteins during the activation of blood platelets (thrombocytes). This causes the platelets to clump together and a blood clot (thrombus) to appear.

  • I am investigating…

    how the proteins’ activation in the thrombocytes changes under reduced oxygen levels compared to normal conditions. It is currently still unclear whether a reduced oxygen concentration activates the thrombocytes and thus leads to thrombosis, or whether it’s the other way around and the activated thrombocytes trigger an oxygen deficiency in the blood.

    To answer this question, the setting in which I conduct my experiments is important. To date, experiments with thrombocytes have mostly been conducted at an oxygen level of 21% - that of the air. In the blood vessels, however, the oxygen content is lower and can drop even further during activation. My work will help to better understand the effect of oxygen deficiency on thrombocytes under real-life conditions.

  • For my bachelor’s thesis, I am conducting research at ISAS because…

    I am interested in analytical chemistry in the context of health research. I was immediately fascinated by the idea of being able to do research on thrombocytes from human samples. I hope that my work can support the scientists at ISAS in identifying makers for an early thrombocyte reaction in patients – and thus ultimately reduce the risk of thrombosis.


Further articles

20th February 2024

SARS-CoV-2: The Very Latest Methods Clarify the Active Agents and the Mechanism of Action of Ancient Self-Medications

Prophylactic, soothing or even healing agents, mostly natural substances, have been known to natural medicine since ancient times. But what about viral infections? Could infusions made from sage or perilla also be used against SARS-CoV-2 infections -as a prevention or an aid to healing? An interdisciplinary team of researchers led by Prof Dr Mirko Trilling from the Faculty of Medicine at the University of Duisburg-Essen (UDE) and scientists at ISAS investigated these questions during the coronavirus pandemic.

Das Bild zeigt Prof. Dr. Mirko Trilling mit verschränkten Armen, an einer Wand lehnend. The picture shows Prof Dr Mirko Trilling with his arms folded, leaning against a wall.
7th February 2024

A Long Sought-After Combination Method in Mass Spectrometry

Researchers who analyse complex samples using mass spectrometers are often faced with the challenge that the substances they contain are fundamentally different. Some are chemically polar, others are non-polar. Until now, this has required two complex separate analyses. But a researcher at ISAS has developed a method with which even less polar substances can be included in a standard mass spectroscopic analysis for polar biological substances.

Daniel Foest steht im Labor und hält ein Papier mit einer Leberprobe, die er am Massenspektrometer untersucht.
12th January 2024

“My research is literally hard work"

Darleen Hüser is looking for the immuno-cellular fingerprint of rheumatoid arthritis. In this interview, the doctoral student reveals her razor-sharp research and why she needs different microscopes.

Das Porträt zeigt ISAS-Doktorandin Darleen Hüser aus der Arbeitsgruppe Bioimaging.
21st December 2023

Science Slam: Humorous Science Communication Is Fun for Everyone

Talking lab equipment, artificial intelligence and expertise from the North Pole - this colourful mix of topics characterised the latest Science Slam at the institute. Four ISAS employees demonstrated with their specialist knowledge and plenty of humour how science communication can be fun for everyone involved.

Luisa Becher fotografiert die vier Teilnehmenden des ISAS Science Slam.
20th December 2023

The Art of Balancing: Accuracy in Image Analysis

What challenges in analysing microscopic images can be mastered with artificial intelligence if the latter is incorporated at an early stage? Why should researchers think about the target metrics of image analysis when planning their experiment? Dr Jianxu Chen has now put together his tips, recently published in the journal Nature Methods, as a kind of checklist for researchers.

Die Abbildung zeigt eine Wage und symbolisiert das Gleichgewicht zwischen Analyse und Genauigkeit bei der Validierung von biomedizinischen Aufnahmen.
1st December 2023

Bone research: ISAS participates in the new "DIONE" Collaborative Research Centre

The nationwide project recently funded by the German Research Foundation is focussing on inflammation-induced bone loss. Researchers from Dortmund, Dresden, Erlangen/Nuremberg and Ulm want to find out exactly how inflammatory diseases - such as rheumatoid arthritis or intestinal diseases - damage bones. Among other things, their research aims to help identify new therapies for skeletal-associated diseases.

Bone structur with osteoporosis.
31st January 2023

ISAS Congratulates Sven Heiles on his Habilitation

With his presentation about analytical chemistry in paleontology at Justus Liebig University (JLU) Giessen, Heiles successfully completed his habilitation.

Prof. Dr. Sven Heiles.
27th January 2023

Guest Presentation: New Framework for Stem Cell Analysis

Dr Susanne Rafelski, deputy director of scientific programme at the Allen Institute for Cell Science is a guest at ISAS on 7 February.

Portrait Susanne Rafelski.
26th January 2023

ISAS Organises 54th DGMS Conference - Registration Now Open

The annual meeting of the German Society for Mass Spectrometry in Dortmund from May 14-17 will focus on mass spectrometric techniques and research.

Bild vom Dortmunder Hafen bei Nacht.