In addition to analysing qualities, the Technical Services Bioanalytics can also quantify proteins. Apart from marker-free quantification, we also determine the relative frequency of proteins in simple and highly complex samples by means of chemically stable isotope labeling on primary amines. We use the commercially available reagent iTRAQ® (isobaric tag for relative and absolute quantification) for this purpose and can thus read out and quantify up to eight samples in parallel. Alternatively, we deploy TMT® (tandem mass tag), which deploys a similar chemical principle and allows up to ten samples to be analysed simultaneously.

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Other services

Ellipsometry of the visible spectrum through to the vacuum ultraviolet range

This method measures the electronic properties, crystallinity and structure of thin layers from the micrometre range through to atomic structures.
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Peptide synthesis and amino acid analysis

Our services include the synthesis of peptide standards for precise and robust protein quantification.
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Polarisation-dependent infrared spectroscopy and ellipsometry

This technique is particularly well suited for investigating new materials, metamaterials, hybrid materials and thin organic and inorganic layers.
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Proteome analysis via LC-MS

ISAS is equipped with the latest LC-MS systems which enable us to reliably identify proteins.
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