The CARS Microscopy junior research group was established in the scope of the Leibniz Research Cluster (LRC). The LRC is a project in which five different Leibniz institutes cooperate with the aim of finding new ways to efficiently produce bioactive components and their preliminary stages. The LRC work groups thus develop and deploy micro reactors in which the reaction conditions for enzymes and other biobricks can be tested, varied and optimised.
The remit of the CARS Microscopy junior work group is to analyse and characterise the processes taking place inside the micro reactors. The composition of minute quantities of samples can already be identified by different techniques such as mass spectrometry, Raman scattering, coherent anti-Stokes Raman scattering (CARS) and other spectroscopic methods, preferably directly on the system and with a high spatial resolution. The group characterises the starting material as well as the by-products and end products, and also analyses the underlying reactions in order to determine whether and how many of the starting material was transformed into the desired product. Subsequent free-flow electrophoresis enables desired and undesired reaction products to be removed from the process and non-transformed starting material to be fed back into the reactor. This allows more of the desired product to be created in pure form.