Redox biochemistry takes a central position in biological processes. Not only enzymes, but also hundreds of small metabolites are electrochemically active, thereby reflecting the redox status of a given biosystem and, even more important, dynamic changes within this system. The most important advantages of electroanalytical methods include an extremely high sensitivity for underivatised (!) compounds, an inherent ‘biocompatibility’ (no need for complicated interfaces), and the availability of several measuring principles - making it an ideal tool for real-time sensing and metabolic profiling at trace levels, as well as for bioelectrochemical modelling and mechanistical investigations, such as DNA-binding, membrane transport, or metal-ligand interactions.
1. Investigation of enzymatic reactions by means of cyclic voltammetry